In vitro study | Berberine chloride hydrate ( Natural Yellow 18 chloride hydrate; 1.25-160 μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29. Berberine chloride hydrate (1.25-160 μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth. LoVo cells are exposure to Berberine chloride hydrate (10-80 μM) for 24 h. Cell cycle analysis of 40 μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase. Berberine chloride hydrate (10-80 μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0 μM. Cell Proliferation Assay Cell Line: Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29 Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM Incubation Time: 72 hours Result: Inhibited the proliferation of four cell lines. The IC 50 ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116). Cell Proliferation Assay Cell Line: Colorectal carcinoma cell lines LoVo Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM Incubation Time: 24, 48, 72 hours Result: Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells. Cell Cycle Analysis Cell Line: LoVo cells Concentration: 0, 10, 20, 40, or 80 μM Incubation Time: 24 hours Result: Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population. Western Blot Analysis Cell Line: LoVo cells Concentration: 10, 20, 40, or 80 μM Incubation Time: 24 hours Result: Suppressed cyclin B1, cdc2 and cdc25c protein expression. |